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All qRT-PCR samples were run in technical duplicates and the Ct method based on the reference value estimate from 3 housekeeping gene (GAPDH, GUSB and TBP) was used to calculate relative expression level of each gene and reaction

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FIGURE 5 FIGURE 6 3.8 Gene expression modulation by SPAE The mRNA expression levels of oxidative stress, apoptosis, lipid metabolism, and DNA damage-related genes were evaluated using quantitative RT-PCR

The problem is that online conversations usually move faster than the clinical evidence, and many patients are left with more excitement than clarity

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